Thermally stable and ph stable subtilisin analogs and method...

Details Thermally stable and ph stable subtilisin analogs and method... Thermally stable and ph stable subtilisin analogs and method...

C - Chemistry, Metallurgy

12

N

195/1.235, 195/3

C12N 15/57 (2006.01) C12N 9/54 (2006.01) C12N 9/56 (2006.01) C12N 15/90 (2006.01)

Patent

CA 1340643

A mutated subtilisin suitable for admixture to washing compositions and exhibiting substantially improved stability over naturally occurring Bacillus serine proteases is prepared by expressing a modified gene encoding subtilisin in Bacillus subtilis. A preferred subtilisin analog product differs from wild-type Bacillus alkaline proteases by having any amino acid, and preferably serine, at position 218 in place of asparagine. The product is preferably produced in a strain of B. subtilis which is mutated to block synthesis of endogenous proteases. The method of replacing an Asn or a Gly in an Asn-Gly sequence in order to improve pH and thermal stability may be applied to other sites in subtilisin and to other proteins as well.

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