Thermocycler and sample vessel for rapid amplification of dna

Details Thermocycler and sample vessel for rapid amplification of dna Thermocycler and sample vessel for rapid amplification of dna

C - Chemistry, Metallurgy

12

M

C12M 1/24 (2006.01) B01L 7/00 (2006.01) C12M 1/38 (2006.01) C12P 19/34 (2006.01)

Patent

CA 2716337

A thermocycler apparatus and method for rapidly performing the PCR process employs at least two thermoelectric modules which are m substantial spatial opposition with an interior space present between opposing modules One or multiple sample vessels are placed m between the modules such that the vessels are subjected to temperature cycling by the modules The sample vessels have a minimal internal dimension that is substantially perpendicular to the modules that facilitates rapid temperature cycling In embodiments of the invention the sample vessels may be deformable between a) a shape having a wide mouth to facilitate filling and removing of sample fluids from the vessel, and b) a shape which is thinner for conforming to the sample cavity or interior space between the thermoelectric modules of the thermocycler for more rapid heat transfer

L'invention concerne un appareil de thermocyclage et un procédé de réalisation rapide de l'opération de PCR, qui utilise au moins deux modules thermoélectriques sensiblement opposés dans l'espace, un espace intérieur se trouvant entre les modules opposés. Un ou plusieurs récipients à échantillons sont placés entre les modules de telle sorte que les récipients soient soumis aux cycles de température par les modules. Les récipients à échantillons ont des dimensions intérieures minimales sensiblement perpendiculaires aux modules qui facilitent un cycle rapide de la température. Dans des modes de réalisation de l'invention, les récipients à échantillons peuvent être déformés a) d'une forme qui présente une large embouchure de manière à faciliter le remplissage et l'élimination des fluides d'échantillon du récipient à b) une forme plus mince qui permet à la cavité ou à l'espace intérieur à échantillons de s'ajuster entre les modules thermoélectriques du thermocycleur pour engendrer un transfert de chaleur plus rapide.

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