C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 9/24 (2006.01) A61K 38/47 (2006.01) A61K 47/30 (2006.01) A61P 35/00 (2006.01) C12N 9/96 (2006.01) C12P 19/34 (2006.01) C12P 21/00 (2006.01) C12S 3/20 (2006.01)
Patent
CA 2238313
The invention concerns a thermolabile enzyme having uracil-DNA-glycosylase activity and characterized in particular by a high degree of purity, short half-life times and a content of less than 2 % contaminating foreign activities. The invention further concerns a process for preparing this enzyme and its use for removing the base uracil from DNA, in particular uracil-containing PCR products. The enzyme can be obtained from Gram-positive microorganisms such as, for example, Arthrobacter or Micrococcus.
L'invention concerne une enzyme thermolabile possédant une activité uracile-ADN-glycosylase et caractérisée notamment par un degré élevé de pureté, des demi-vies courtes et une teneur en activités étrangères contaminantes inférieure à 2 %. L'invention concerne également un procédé pour préparer cette enzyme et son utilisation pour éliminer la base uracile de l'ADN, notamment les produits de la PCR contenant de l'uracile. Cette enzyme s'obtient à partir de micro-organismes Gram-positif tels que, par exemple, Arthrobacter ou Micrococcus.
Frey Bruno
Kaluza Klaus
Schmidt Manfred
Sobek Harald
Boehringer Mannheim Gmbh
Norton Rose Or S.e.n.c.r.l. S.r.l./llp
Roche Diagnostics Gmbh
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