Thermophilic dna polymerases from thermoactinomyces vulgaris

C - Chemistry – Metallurgy – 12 – N

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C12N 15/54 (2006.01) C07H 21/04 (2006.01) C12N 1/21 (2006.01) C12N 5/10 (2006.01) C12N 9/12 (2006.01) C12N 15/63 (2006.01) C12P 19/34 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2401727

The present invention provides compositions comprising thermostable DNA polymerases derived from hyperthermophilic eubacteria. In particular, the present invention comprises thermostable DNA polymerases from the hyperthermophilic eubacterial species Thermoactinomyces vulgaris. The present invention also provides methods for utilizing naturally-occurring and non-naturally-occurring forms of T. vulgaris DNA polymerase in sequencing, reverse transcription, and amplification reactions.

L'invention concerne des compositions contenant des ADN polymérases thermostables dérivées d'eubactéries hyperthermophiles. L'invention concerne notamment des ADN polymérases thermostables dérivées de l'espèce eubactérienne hyperthermophile <i>Thermoactinomyces vulgaris</i>. L'invention concerne également des méthodes d'utilisation de formes naturelles et non naturelles d'ADN polymérases de <i>T. vulgaris</i> pour effectuer des séquençages, des transcriptions inverses et des réactions d'amplification.

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