Thermoresistant alpha-1-antitrypsin mutein

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/15 (2006.01) C07K 14/81 (2006.01) C12N 1/19 (2006.01) C12N 1/21 (2006.01)

Patent

CA 2163081

The mutagenized recombinant AT of the present invention wherein same amino acids of the amino acid sequence of a wild-type AT are replaced with another amino acid has all enhanced thermoresistance compared to the wild-type AT, while maintaining its activity. A vector containing a gene encoding the recombinant AT, a microorganism transformed with said vector and a process for producing the recombinant AT with a higher thermoresistance using said microorganism are also disclosed.

L'invention concerne une mutéine thermorésistante d'alpha-1-antitrypsine (AT) obtenue par mutagénèse, dans laquelle quelques acides aminés de la séquence aminoacide d'une AT de type sauvage sont remplacés par un autre acide aminé et qui présente une thermorésistance améliorée par rapport à l'AT de type sauvage, tout en conservant son efficacité. L'invention décrit également un vecteur contenant un gène codant l'AT recombinante, un microorganisme transformé au moyen dudit vecteur et un procédé de préparation de l'AT recombinante, présentant une thermorésistance supérieure au moyen dudit microorganisme.

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