C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/55 (2006.01) A61K 31/505 (2006.01) A61K 38/46 (2006.01) A61K 47/12 (2006.01) A61K 47/16 (2006.01) A61K 47/22 (2006.01) A61P 25/36 (2006.01) C12N 9/16 (2006.01) C12Q 1/00 (2006.01) C12Q 1/37 (2006.01) C12Q 1/44 (2006.01) G01N 33/68 (2006.01)
Patent
CA 2691842
Provided are compositions comprising a cocaine esterase (CocE) and a compound that thermostabilizes the CocE. Also provided are methods of thermostabilizing a cocaine esterase. Additionally provided are methods of treating a mammal undergoing a cocaine-induced condition. Methods of determining whether a compound is a thermostabilizing agent for a protein are also provided. Uses of the above-described compositions for the treatment of a cocaine-induced condition is additionally provided. Additionally provided is an isolated nucleic acid encoding a CocE polypeptide having the substitutions L169K and G173Q, and the CocE polypeptide encoded by that nucleic acid, and pharmaceutical compositions thereof. Further provided is the use of that composition for the manufacture of a medicament for the treatment of a cocaine-induced condition and for the treatment of a cocaine-induced condition.
L'invention concerne des compositions comprenant une cocaïne estérase (CocE) et un composé qui thermostabilise la CocE. Des procédés pour thermostabiliser une cocaïne estérase sont également proposés ainsi que des procédés pour traiter un mammifère atteint d'une affection induite par la cocaïne. L'invention concerne également des procédés permettant de déterminer si un composé est un agent thermostabilisant pour une protéine ou pas. Des utilisations des compositions décrites ci-dessus pour traiter une affection induite par la cocaïne sont proposées. L'invention concerne en plus un acide nucléique isolé codant pour un polypeptide CocE portant les substitutions L169K et G173Q, et le polypeptide CocE codé par cet acide nucléique, des compositions pharmaceutiques les contenant, ainsi que l'utilisation de ces compositions dans la fabrication d'un médicament destiné à traiter une affection induite par la cocaïne.
Brim Remy L.
Landry Donald
Macdonald Joanne
Narasimhan Diwahar
Stojanovic Milan N.
Kirby Eades Gale Baker
The Regents Of The University Of Michigan
The Trustees Of Columbia University In The City Of New York
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