Thermostable asparaginases

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 9/82 (2006.01) A23L 1/10 (2006.01) A23L 1/214 (2006.01) C12N 15/52 (2006.01)

Patent

CA 2680273

The invention relates to new asparaginases having improved properties, preferably improved thermotolerance, such as improved activity at high temperatures and/or improved thermostability. The invention also relates to DNA sequences encoding such improved asparaginases, their production in a recombinant host cell, as well as methods of using the asparaginases, in particular for reduction of acrylamide in foods. The invention furthermore relates to methods of generating and preparing asparaginase variants having improved properties.

L'invention porte sur de nouvelles asparaginases ayant des propriétés améliorées, de préférence, une thermotolérance améliorée, telle qu'une activité améliorée à des températures élevées et/ou une thermostabilité améliorée. L'invention concerne également des séquences d'ADN codant pour de telles asparaginases améliorées, leur production dans une cellule hôte recombinante, ainsi que des procédés d'utilisation des asparaginases, en particulier pour la réduction de l'acrylamide dans les aliments. L'invention concerne de plus des procédés consistant à générer et à préparer des variants d'asparaginase ayant des propriétés améliorés.

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