C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/54 (2006.01) C12N 1/21 (2006.01) C12N 9/10 (2006.01) C12N 15/70 (2006.01) C12P 19/34 (2006.01) C07K 1/16 (2006.01)
Patent
CA 2268014
A DNA polymerase from a thermophilic eubacterium is provided. The DNA polymerase shows magnesium ion dependent reverse transcriptase activity and 3'-5' exonuclease activity. The invention also includes recombinant plasmids and transformed host cells capable of producing the enzyme. The enzyme is classified into class EC 2.7.7.7., a DNA nucleotidyl transferase DNA-directed type.
L'invention concerne une polymérase d'ADN provenant d'une eubactérie thermophile. La polymérase d'ADN présente une activité de transcriptase inverse dépendant des ions de magnésium et une activité d'exonucléase en 3'-5'. L'invention traite également de plasmides de recombinaison et de cellules hôtes transformées permettant de produire cette enzyme. Cette enzyme est classée en classe EC 2.7.7.7., de type ciblé sur l'ADN de transférase de nucléotidyle d'ADN.
Angerer Bernhard
Ankenbauer Waltraud
Bonch-Osmolovskaya Elizaveta
Ebenbichler Christine
Laue Frank
Norton Rose Or S.e.n.c.r.l. S.r.l./llp
Roche Diagnostics Gmbh
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