Thermostable dna polymerases

Details Thermostable dna polymerases Thermostable dna polymerases

C - Chemistry, Metallurgy

12

N

C12N 15/54 (2006.01) C12M 1/40 (2006.01) C12N 9/12 (2006.01) C12Q 1/68 (2006.01) C12P 19/34 (2006.01)

Patent

CA 2222744

An enzymatically active DNA polymerase having between 540 and 582 amino acids having a tyrosine at a position equivalent to position 667 of Taq DNA polymerase, wherein said polymerase lacks 5' to 3' exonuclease activity, and wherein said polymerase has at least 95 % homology in its amino acid sequence to the DNA polymerase of $(i)(Thermus aquaticus), $(i)(Thermus flavus) or $(i)(Thermus thermophilus), and wherein said polymerase forms a single polypeptide band on an SDS PAGE.

ADN-polymérase à activité enzymatique comportant entre 540 et 582 acides aminés, avec une tyrosine dans une position équivalente à la position 667 de l'ADN-polymérase Taq. Cette polymérase ne présente pas d'activité d'exonucléase en position 5' à 3', et présente une homologie d'au moins 95 % dans sa séquence d'acides aminés par rapport à l'ADN polymérase de Thermus aquaticus, Thermus flavus ou Thermus thermophilus, et elle forme une seule bande polypeptidique sur SDS PAGE.

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