Thermostable enzyme promoting the fidelity of thermostable...

Details Thermostable enzyme promoting the fidelity of thermostable... Thermostable enzyme promoting the fidelity of thermostable...

C - Chemistry, Metallurgy

12

N

C12N 15/55 (2006.01) C12N 9/12 (2006.01) C12N 9/22 (2006.01) C12N 15/10 (2006.01) C12N 15/54 (2006.01) C12P 19/34 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2351634

A purified thermostable enzyme is derived from the thermophilic archaebacterium Archaeoglobus fulgidus. The enzyme can be native or recombinant, is stable under PCR conditions and exhibits double strand specific exonuclease activity. It is a 3'-5' exonuclease and cleaves to produce 5'-mononucleotides. Thermostable exonucleases are useful in many recombinant DNA techniques, in combination with a thermostable DNA polymerase like Tag especially for nucleic acid amplification by the polymerase chain reaction (PCR).

La présente invention concerne une enzyme thermostable purifiée dérivée de l'<i>archaebacterium</i> thermophile <i>Archaeoglobus fulgidus</i>. L'enzyme peut être une enzyme native ou recombinée, est stable en conditions de PCR et présente une activité d'exonucléase spécifique à double brin. Il s'agit d'une exonucléase 3'-5' qui se coupe pour produire des mononucléotides 5'. Les exonucléases thermostables sont utiles dans de nombreuses techniques de recombinaison d'ADN, en combinaison avec une polymérase d'ADN thermostable telle que <i>Taq</i> en particulier pour l'amplification d'acide nucléique par réaction en chaîne polymérase (<i>polymerase chain reaction</i> / PCR).

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