Thermostable luciferases and methods of production

Details Thermostable luciferases and methods of production Thermostable luciferases and methods of production

C - Chemistry, Metallurgy

12

N

C12N 15/53 (2006.01) C12N 9/02 (2006.01) C12N 15/10 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2301828

Luciferase enzymes with greatly increased thermostability, e.g., at least half lives of 2 hours at 50 ~C, cDNAs encoding the novel luciferases, and hosts transformed to express the luciferases, are disclosed. Methods of producing the luciferases include recursive mutagenesis. The luciferases are used in conventional methods, some employing kits.

Enzymes luciférases présentant une stabilité thermique considérablement améliorée, par exemple, des demi-vies de 2 heures à 50·C, ADNc codant ces nouvelles luciférases et hôtes transformés afin d'exprimer ces luciférases. Procédés servant à produire ces luciférases notamment par mutagenèse récursive. On utilise ces luciférases dans des procédés classiques dont certains mettent en application des trousses.

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