Thermostable luciferases from photuris pennsylvanica and...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/53 (2006.01) C12N 9/02 (2006.01) C12N 15/10 (2006.01)

Patent

CA 2350823

Luciferase enzymes with greatly increased thermostability, e.g., at least half lives of 2 hours at 50 ~C, cDNAs encoding the novel luciferases, and hosts transformed to express the luciferases, are disclosed. Methods of producing the luciferases include recursive mutagenesis. The luciferases are used in conventional methods, some employing kits.

L'invention concerne des enzymes luciférases présentant une stabilité thermique considérablement améliorée, par exemple, des demi-vies de 2 heures à 50 ·C, des ADNc codant pour ces nouvelles luciférases et des hôtes transformés afin d'exprimer ces luciférases. L'invention concerne également des procédés servant à produire ces luciférases notamment par mutagenèse récursive. Ces luciférases sont utilisées dans des procédés classiques dont certains mettent en application des trousses.

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