Thermostable non-reducing saccharide-forming enzyme, its...

C - Chemistry – Metallurgy – 12 – N

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C12N 9/24 (2006.01) A23L 1/10 (2006.01) A23L 1/22 (2006.01) A61K 8/60 (2006.01) A61K 31/70 (2006.01) A61K 47/26 (2006.01) C07H 3/04 (2006.01) C12P 19/12 (2006.01) C12P 19/14 (2006.01) A61K 7/00 (1995.01)

Patent

CA 2151219

Disclosed are novel thermostable non-reducing saccharides-forming enzyme, its preparation and uses. The enzyme is obtainable from the culture of microorganisms such as Sulfolobus acidocaldarius (ATCC 33909 and ATCC 49426) and Sulfolobus solfataricus (ATCC 35091 and ATCC 35092), and capable of forming non-reducing saccharides having a trehalose structure as an end unit when allowed to act on reducing partial starch hydrolysates at a temperature of over 55°C. Glucoamylase and .alpha.--glucosidase readily yield trehalose when allowed to act on the non-reducing saccharides. These non-reducing saccharides and trehalose are extensively useful in food products, cosmetics and pharmaceuticals.

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