Thermostable nucleoside diphosphate kinase for nucleic acid...

Details Thermostable nucleoside diphosphate kinase for nucleic acid... Thermostable nucleoside diphosphate kinase for nucleic acid...

C - Chemistry, Metallurgy

12

N

C12N 9/12 (2006.01) C12N 1/21 (2006.01) C12N 15/54 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2359070

A thermostable nucleoside diphosphate kinase (NDPK) enzyme useful in a process for the detection of nucleic acid is disclosed. The enzyme, its variants and analogs exhibit higher NDPK activity at a temperature of about 50 ~C to about 90 ~C relative to NDPK activity at 37 ~C. Methods of obtaining, preparing and using the enzyme are also disclosed.

Enzyme nucléoside diphosphate kinase (NDPK) thermostable utile pour détecter des acides nucléiques. Cet enzyme, ses variantes et ses analogues présentent une activité supérieure de NDPK à une température de 50·C à 90 ·C par rapport à l'activité de NDPK à 37 ·C. Procédés d'obtention, de préparation et d'utilisation de cet enzyme.

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