Thermostable proteinases from thermophilic bacteria

Details Thermostable proteinases from thermophilic bacteria Thermostable proteinases from thermophilic bacteria

C - Chemistry, Metallurgy

12

Q

C12Q 1/37 (2006.01) C12N 1/20 (2006.01) C12N 9/52 (2006.01) C12N 9/54 (2006.01) C12N 9/64 (2006.01)

Patent

CA 2447258

The present invention relates to thermostable proteinases from thermophilic Bacillus species and their uses in the preparation of nucleic acid samples. The enzymes of the invention are stable and active at 65-80 ~C, but are readily autolysed or denatured above 90 ~C.

La présente invention se rapporte à des protéinases thermostables provenant d'espèces thermophiles de type Bacillus ainsi qu'à leur utilisation pour la préparation d'échantillons d'acides nucléiques. Les enzymes de la présente invention sont stables et actives aux températures comprises entre 65 et 80 ·C, mais elles sont facilement autolysées ou dénaturées au-dessus de 90 ·C.

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