Thermostable taq polymerase fragment

Details Thermostable taq polymerase fragment Thermostable taq polymerase fragment

C - Chemistry, Metallurgy

12

N

C12N 15/54 (2006.01) A61K 38/45 (2006.01) C07H 21/04 (2006.01) C07K 19/00 (2006.01) C11D 3/386 (2006.01) C12N 1/15 (2006.01) C12N 1/19 (2006.01) C12N 1/21 (2006.01) C12N 5/10 (2006.01) C12N 9/00 (2006.01) C12N 9/12 (2006.01) C12N 9/22 (2006.01) C12N 15/00 (2006.01) C12N 15/09 (2006.01) C12N 15/11 (2006.01) C12N 15/52 (2006.01) C12N 15/62 (2006.01) C12N 15/63 (2006.01) C12P 21/02 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2474734

It was found that a fragment of native Thermus aquaticus DNA polymerase (TaqWT) lacking 288 N-terminal amino acids (Taq.DELTA.288) possesses an increased thermostability over TaqWT, Taq.DELTA.279, and Taq.DELTA.289. The present invention therefore provides Taq.DELTA.288, recombinant expression vectors encoding the same or derivatives thereof, as well as purification protocols for Taq.DELTA.288. The invention also encompasses kits containing Taq.DELTA.288 as well as the use of Taq.DELTA.288 and kits containing Taq.DELTA.288. In addition, the invention encompasses methods for the sequencing a nucleic acid template and methods for amplifying a target nucleic acid.

Il a été établi qu'un fragment d'ADN polymérase (TaqWT) de Thermus aquaticus indigène dépourvu de 288 acides aminés N-terminaux (Taq.DELTA.288) possède une plus grande thermostabilité par rapport à TaqWT, Taq.DELTA.279 et Taq.DELTA.289. La présente invention a pour objet Taq.DELTA.288, des vecteurs d'expression recombinants codant celui-ci ou des dérivés de celui-ci, ainsi que des protocoles de purification de Taq.DELTA.288. La présente invention a aussi pour objet des trousses contenant le Taq.DELTA.288 et l'utilisation de Taq.DELTA.288 ainsi que des trousses qui en contiennent. De plus, la présente invention a pour objet des méthodes de séquençage d'une matrice d'acides nucléiques et des méthodes d'amplification d'un acide nucléique cible.

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