Thermostable trehalose-releasing enzyme, and its preparation...

C - Chemistry – Metallurgy – 12 – N

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C12N 9/24 (2006.01) A23L 1/22 (2006.01) A61K 31/70 (2006.01) C07H 3/06 (2006.01) C12P 19/12 (2006.01) C12P 19/14 (2006.01) A61K 7/00 (1995.01)

Patent

CA 2151438

Disclosed are novel thermostable trehalose-releasing enzyme, and its preparations and uses. The enzyme is obtainable from the culture of microorganisms such as Sulfolobus acidocaldarius (ATCC 33909 and ATCC 49426) and Sulfolobus solfataricus (ATCC 35091 and ATCC 35092), and capable of hydrolyzing at a temperature of over 55°C the linkage between a trehalose moiety and the remaining glycosyl moiety in a non-reducing saccharide having a trehalose structure as an end unit and having a degree of glucose polymerization of 3 or higher. Trehalose and compositions containing the same are extensively useful in food products, cosmetics and pharmaceuticals.

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