Thermostable viral polymerases and methods of use

Details Thermostable viral polymerases and methods of use Thermostable viral polymerases and methods of use

C - Chemistry, Metallurgy

12

N

C12N 9/12 (2006.01) C07K 14/005 (2006.01) C07K 14/02 (2006.01)

Patent

CA 2624324

Thermostable viral polymerases exhibiting a combination of activities selected from, proofreading (3'-5') exonuclease activity, nick translating (5'-3') nuclease activity, synthetic primer-initiated polymerase activity, nick- initiated polymerase activity, reverse transcriptase activity, strand displacement activity, and/or decreased discrimination against incorporation of nucleotide analogs. Also provided are compositions including the polymerases, polynucleotides encoding the polymerases and methods of using the polymerases.

L'invention porte sur des polymérases virales thermostables présentant la combinaison des activités: d'exonucléase de correction d'épreuve (3'-5'), de nucléase de translation de coupure (5'-3'), de polymérase produite par amorce de synthèse, de polymérase produite par translation de coupure, de transcriptase inverse, de déplacement de brins, et/ou de moindre discrimination contre l'incorporation d'analogues de nucléotides. L'invention porte également sur des compositions contenant les polymérases, et les polynucléotides codant pour les polymérases, et sur des méthodes d'utilisation des polymérases.

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