Thermostable xylanases

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 9/24 (2006.01) C12S 3/08 (2006.01) D21C 5/00 (2006.01) D21C 9/10 (2006.01)

Patent

CA 2188874

The present invention relates to novel xylanases purified from the microorganism, Microtetraspora flexuosa. Each biochemi- cally unique xylanase is thermostable and has optimal activity in alkaline conditions. The present invention is also directed to methods that utilize either the whole enzyme system or one or more of the above purified ther- mostable xylanases obtained from Microte- traspora flexuosa. These enzymes are ex- cellent candidates for enhancing the deligni- fication and bleaching of pulp. Furthermore, treating the pulp with the above enzymes prior to bleaching may reduce the amount of chlo- rine containing and or peroxide chemicals re- quired in the bleaching process.

La présente invention se rapporte à de nouvelles xylanases purifiées à partir du micro-organisme Microtetraspora flexuosa. Chaque xylanase biochimiquement unique est thermostable et présente une activité optimale dans des conditions alcalines. La présente invention se rapporte également à des procédés faisant appel soit au système enzymatique complet soit à au moins une des xylanases thermostables purifiées précitées obtenues à partir du Microtetraspora flexuosa. Ces enzymes sont d'excellents agents potentiels pour améliorer la délignification et le blanchiment de pâte à papier. En outre, le traitement de la pâte au moyen desdites enzymes, avant le blanchiment, permet de réduire la quantité de produits chimiques au peroxyde et/ou contenant du chlore requis pour le processus de blanchiment.

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