Three dimensional cell culture

Details Three dimensional cell culture Three dimensional cell culture

C - Chemistry, Metallurgy

12

N

C12N 5/06 (2006.01)

Patent

CA 2648361

A method for culturing preadipocytes isolated ex vivo is described, the method including introducing preadipocytes into a three dimensional support matrix, and allowing the cells to differentiate in vitro into adipocytes within the support matrix. The matrix may be a collagen matrix. The method may be used for investigating the development of stem cells, or for investigating the response of adipocytes to stimuli. The method provides a system whereby adipocytes with biological properties resembling those in vivo can be grown in vitro.

La présente invention concerne un procédé de culture de pré-adipocytes isolés ex vivo, le procédé consistant à introduire des pré-adipocytes dans un support matriciel tridimensionnel, et à permettre aux cellules de se différencier in vitro pour former lesdits adipocytes dans le support matriciel. La matrice peut être une matrice de collagène. Le procédé peut être utilisé pour étudier le développement de cellules souches, ou pour étudier la réponse d'adipocytes à un stimulus. Le système du présent procédé permet de cultiver in vitro des adipocytes présentant des propriétés biologiques semblables à celles in vivo.

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