A - Human Necessities – 61 – F
Patent
A - Human Necessities
61
F
A61F 2/24 (2006.01) A61L 27/38 (2006.01) A61F 2/06 (2006.01)
Patent
CA 2199810
The present invention relates to a method of growing a variety of different cells and tissues in three-dimensional cultures in vitro using human fibroblasts in the culture medium. In accordance with the invention, stromal cells, including but not limited to human dermal and cardiac fibroblasts, are inoculated and grown on a three-dimensional scaffold or framework. The human fibroblasts secrete human matrix proteins to supplement and replace the existing porcine matrix composed of decellularized heart valves or aortic walls and leaflets formed into three-dimensional constructs having interstitial spaces bridged by the stromal cells. The living stromal tissue so formed provides the support, growth factors, and regulatory factors necessary to sustain long-term viability and proliferation of cells in culture and/or cultures implanted in vivo. When grown in this three-dimensional system, the proliferating cells mature and segregate properly to form components of tissues analogous to counterparts in vivo.
L'invention concerne un procédé qui permet de faire croître différents tissus et cellules dans des cultures tridimensionnelles in vitro en utilisant des fibroblastes humains dans des milieux de culture. Des cellules du stroma, comprenant notamment des fibroblastes dermiques et cardiaques humains, sont inoculés et croissent sur une grille ou structure tridimensionnellle. Ces fibroblastes humains secrètent des protéines de matrice humaine destinées aà compléter et remplacer la matrice porcine existante composée de valvules cardiaques ou de parois et valves aortiques privées de leurs cellules et prenant la forme de reconstructions tridimensionnelles dotées d'espaces intersticiels reliés par les cellules du stroma. Le tissu du stroma vivant ainsi formé fournit un support ainsi que des facteurs de croissance et de régulation nécessaires pour assurer la viabilité et la prolifération à long terme des cellules mises en culture et/ou implantées in vivo. Lorsqu'elles croissent sur cette structure tridimensionnelle, les cellules qui prolifèrent deviennent matures et se différencient nettement pour former des composants de tissus analogues à leur équivalents in vivo.
Campbell Todd D.
Landeen Lee K.
Naughton Brian A.
Naughton Gail K.
Purchio Anthony F.
Advanced Tissue Sciences Inc.
Osler Hoskin & Harcourt Llp
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