Three-dimensional mammalian ovarian follicular cell and...

A - Human Necessities – 61 – K

Patent

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A61K 9/50 (2006.01) C12N 5/08 (2006.01)

Patent

CA 2558306

A process for encapsulating and immobilising mammalian stem cells, ovarian follicular cells, gametes, ovarian follicles or mammalian embryos which are able to auto-organise into three-dimensional structures in vitro, and express biological functions in a manner similar to that which is observed in the organism in vivo is described. The capsules are constituted by: a nucleus containing stem cells, ovarian follicular cells, gametes, ovarian follicles or mammalian embryos and/or a biocompatible and/or biodegradable polymer; a semi- permeable membrane constituted by a divalent or trivalent metal ion salt of alginic acid, optionally cross-linked on the inner and/or outer surface and/or on both surfaces, optionally vehicularising a second or more cellular species. The cells and follicles prepared and cultivated using this methodology are used for the in vitro and/or in vivo production of peptides, proteins, antibodies, hormones and hormone precursors, metabolites and catabolites typical of these cellular structures, plasma membranes, nuclear membranes, cytoplasmic organelles, somatic cell nuclei or gametes and embryos.

L'invention porte sur un procédé d'encapsulation et d'immobilisation de cellules souches mammaliennes, de cellules folliculaires ovariennes, de gamètes, de follicules ovariens ou embryons mammaliens qui sont capables de s'auto-organiser en structures tridimensionnelles in vitro et d'exprimer des fonctions biologiques d'une manière similaire à celle que l'on peut observer dans l'organisme in vivo. Les capsules sont constituées d'un noyau contenant des cellules souches, de cellules folliculaires ovariennes, de gamètes, de follicules ovariens ou d'embryons mammaliens et/ou d'un polymère biocompatible et/ou biodégradable; d'une membrane semi-perméable constituée d'un sel d'ion métallique divalent ou trivalent d'acide alginique, éventuellement réticulé sur la surface interne et/ou externe et/ou sur les deux surfaces, éventuellement véhiculariser une seconde ou plusieurs espèces cellulaires. Les cellules et les follicules préparés et cultivés selon cette méthodologie sont utilisés dans la production in vitro et/ou in vivo de peptides, de protéines, d'anticorps, d'hormones et de précurseurs hormonaux, de métabolites et de catabolites typiques de ces structures cellulaires, des membranes plasmiques, de membranes nucléaires, d'organelles cytoplasmiques, de noyaux cellulaires somatiques ou de gamètes et d'embryons.

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