C - Chemistry – Metallurgy – 12 – M
Patent
C - Chemistry, Metallurgy
12
M
C12M 3/00 (2006.01) C12M 1/22 (2006.01) C12M 1/34 (2006.01) C12N 5/06 (2006.01)
Patent
CA 2398084
The invention relates to a device for producing a three-dimensional circular muscle body from a carrier substance and from cell cultures incorporated therein comprising a cell culture dish (1) and at least one element (2) placed inside said cell culture dish (1). The invention also relates to a multi-well plate constructed of a number of devices, which are arranged side by side and/or one behind the other, for producing a three-dimensional circular muscle body. The invention additionally relates to a solution for cultivating mammal cardiomyocytes, to a method for cultivating a cell culture, to a device for measuring isometric force parameters of cell cultures, and to a method for following, in a measurable manner, contractions of a cell tissue incorporated in a carrier substance. Finally, the invention relates to an artificially produced three-dimensional muscle tissue, in particular, a heart muscle tissue.
L'invention concerne un dispositif permettant de produire un corps musculaire circulaire tridimensionnel, à partir d'une substance porteuse et de cultures cellulaires incorporées dedans. Ledit dispositif comprend un récipient de culture cellulaire (1) et au moins un élément (2) placé dans le récipient de culture cellulaire (1). L'invention concerne en outre une plaquette à puits multiples qui se compose de plusieurs dispositifs disposés les uns à côté des autres et/ou les uns derrière les autres, s'utilisant pour produire un corps musculaire circulaire tridimensionnel. L'invention concerne par ailleurs une solution de mise en culture de cardiomyocytes de mammifères et un procédé de mise en culture d'une culture cellulaire. L'invention concerne également un dispositif permettant de mesurer les paramètres de force isométriques de cultures cellulaires, ainsi qu'un procédé de suivi mesurable de contractions d'un tissu cellulaire incorporé dans une substance porteuse. L'invention concerne pour finir un tissu musculaire tridimensionnel produit de manière artificielle, notamment un tissu musculaire cardiaque.
Borden Ladner Gervais Llp
Medigene Ag
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