Three-dimensional physiological matrices for oncological...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/02 (2006.01) G01N 33/50 (2006.01) C12N 5/071 (2010.01) C12M 1/34 (2006.01)

Patent

CA 2698338

Three-dimensional physiological matrices, methods, apparatus and kits for the expedited design, testing and evaluation of oncological remedies are provided. Key aspects of the inventions include matrices, and especially gel matrices, comprising one or more physiological fibers, which are adapted and arranged to provide conditions which permit behaviors, such as the movement of cells away from the margins of samples of target tissue through the matrix, to be evaluated in a manner that produces data useful for evaluating the oncological status and characteristics of the cells. In a further key aspect, the invention permits the in vitro testing and analyses of one or more conventional, experimental or theoretical therapies with respect to specific target tissues or cells. Among such therapies are therapeutic compounds and combinations thereof, radiation therapies, combinations of therapeutic compounds and radiation and numerous other possible therapies.

L'invention concerne des matrices physiologiques tridimensionnelles, des procédés, un appareil et des kits pour la l'élaboration, le test et l'évaluation rapide de médications oncologiques. Les matrices constituent l'aspect clé de l'invention, notamment les matrices en gel, incluant une ou plusieurs fibres physiologiques, adaptées et agencées pour fournir des conditions permettant d'évaluer des comportements, tels que le l'éloignement de cellules des marges des échantillons de tissu cible à travers la matrice, et d'obtenir des données utiles pour l'évaluation de l'état oncologique et des caractéristiques des cellules. Dans un autre aspect clé, l'invention permet de tester in vitro et d'analyser une ou plusieurs thérapies classiques, expérimentales ou théoriques sur des tissus ou des cellules cibles spécifiques. De telles thérapies incluent des composés thérapeutiques et des combinaisons de ceux-ci, des thérapies par rayonnement, des combinaisons de composés thérapeutiques et par rayonnement ainsi que de nombreuses autres applications possibles.

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