G - Physics – 01 – B
Patent
G - Physics
01
B
G01B 11/00 (2006.01) G01N 21/64 (2006.01) G02B 21/00 (2006.01)
Patent
CA 2614254
[PROBLEMS] To provide three dimensional position observation method and apparatus for three-dimensionally detecting a position of an observation object and also for contributing to high precision detection of three- dimensional motion of one protein molecule moving under a microscope. [MEANS FOR SOLVING PROBLEMS] The observation apparatus is provided with a lens system having a focusing and diaphragm mechanisms and an image is formed on an imaging plane by light from the observation object. In the observation apparatus, a polarizing member for changing the traveling direction of the observation light into a plurality of different directions is provided in a light path reaching the imaging plane from the observation object, and the position of the observation object is analyzed by an image analyzing means, based on a positional relationship between a plurality of images reached the imaging plane through the polarizing member. A polarizing member which changes a part of the traveling direction of the observation light may be arranged in the light path reaching the imaging plane from the observation object, and the position of the observation object may be analyzed based on the positional relationship between an image reached the imaging plane through the polarizing member and an image reached the imaging plane not through the polarizing member by means of the image analyzing means.
L~invention porte sur un procédé et appareil d~observation de position en trois dimensions pour détecter en trois dimensions une position d~un objet d~observation et également pour contribuer à une détection de grande précision de mouvement en trois dimensions d~une molécule de protéine se déplaçant sous un microscope. L~invention concerne un appareil d~observation muni d~un système de lentille ayant des mécanismes de focalisation et de membrane. Une image est formée sur un plan d~imagerie par une lumière provenant de l~objet d~observation. L~appareil d~observation comporte un élément de polarisation permettant de changer la direction de déplacement de la lumière d~observation dans une pluralité de différentes directions dans un trajet lumineux atteignant le plan d~imagerie depuis l~objet d~observation, et la position de l~objet d~observation est analysée par un moyen d~analyse d~image, sur la base de la relation positionnelle entre une pluralité d~images ayant atteint le plan d~imagerie à travers l~élément de polarisation. Un élément de polarisation qui modifie une partie de la direction de déplacement de la lumière d~observation peut être disposé dans le trajet lumineux atteignant le plan d~imagerie depuis l~objet d~observation, et la position de l~objet d~observation peut être analysée sur la base de la relation positionnelle entre une image ayant atteint le plan d~imagerie à travers l~élément de polarisation et une image ayant atteint le plan d~imagerie autrement qu'à travers l~élément de polarisation grâce au moyen d~analyse d~image.
Mizutani Kana
Nishizaka Takayuki
Japan Science And Technology Agency
Ridout & Maybee Llp
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