Thrombotic episode risk assays using oxidized phospholipids

G - Physics – 01 – N

Patent

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G01N 33/86 (2006.01)

Patent

CA 2385012

An assay to assess thrombotic risk in which oxidized lipids comprising phospholipids are utilized as a membrane source in a clotting assay and the results compared to an assay in which unoxidized phospholipid is used as a membrane source in the presence and absence of activated protein C ("APC"). The assay can monitor for the presence of antibodies in the patient which interfere specifically with the anticoagulant function of APC in an oxidation dependent or independent manner. This can indicate the propensity of the patient to experience episodes of vein thrombosis or arterial thrombosis.

L'invention concerne des essais relatifs à l'évaluation de risque d'épisode thrombotique, qui reposent sur l'utilisation de lipides oxydés renfermant des phospholipides comme source de membrane, en essai de coagulation. On compare les résultats avec ceux d'un essai dans lequel des phospholipides sont utilisés comme source de membrane avec et sans protéine C activée ("APC"). On peut ainsi contrôler chez un patient la présence d'anticorps qui interfèrent spécifiquement avec la fonction d'anticoagulant de l'APC, en scénario de dépendance ou d'indépendance par rapport à l'oxydation. Il en découle une indication sur les risques encourus par le patient quant à la survenue d'épisodes thrombotiques veineux ou artériels.

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