C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
150/8.5
C12Q 1/68 (2006.01) G01N 33/533 (2006.01)
Patent
CA 1330030
TIME-RESOLVED FLUORIMETRIC DETECTION OF LANTHANIDE LABELED NUCLEOTIDES ABSTRACT OF THE DISCLOSURE Nucleotides conjugated with a chelating agent and labeled with a lanthanide, especially terbium, are readily detected by time-resolved fluorimetry during and following gel electrophoresis. The labeled nucleotide conjugates are very stable, highly fluorescent with a long radiative lifetime and remain fluorescent during dilution and during gel electrophoresis and since an enhancement solution is not required for detection, the labeled nucleotide conjugates can be detected on-line, if desired.
598102
Picozza Enrico G.
Saavedra Steven S.
Osler Hoskin & Harcourt Llp
Perkin-Elmer Corporation The
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