Time-stable liquid cholesterol assay compositions

Details Time-stable liquid cholesterol assay compositions Time-stable liquid cholesterol assay compositions

C - Chemistry, Metallurgy

12

Q

150/15, 150/3.3

C12Q 1/60 (2006.01)

Patent

CA 1205364

-30- IMPROVED TIME-STABLE LIQUID CHOLESTEROL ASSAY COMPOSITIONS Abstract of the Disclosure There is provided a stable cholesterol assay composi- tion which comprises an aqueous solution of at least one bile acid or salt thereof being present in an amount of up to about 5mM; a nonionic surfactant present in an amount of from about 0.15 to about 1.5 percent volume by volume; a buffer in a concentration of from 0 to about 65 mM; and cholesterol oxidase in a concentration of at least about 0.2 KIU/l. Solution pH is from about 5.5 to about 8.5. Addition of cholesterol esterase, phenol, peroxidase and 4-aminoantipyrine provides a total cholesterol chromogen system.

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