Tissue culture medium

Details Tissue culture medium Tissue culture medium

C - Chemistry, Metallurgy

12

N

195/43, 195/45,

C12N 5/02 (2006.01) C12N 1/38 (2006.01) C12N 5/00 (2006.01)

Patent

CA 1215335

-45- Tissue Culture Medium Abstract A growth factor containing serum derived from natural bovine serum by precipitating a first solids fraction from natural bovine serum by adding ammonium sulfate to about 25% saturation to give a first solids fraction and a first supernatant; separating said first solids fraction from said first supernatant; precipitating a second solids fractions from said first sypernatant by adding ammonium sulfate to about 40% saturation to give a second solids fraction and a second spernatant; separating said second solids fraction from said second supernatant; combining said first solids fraction and said second supernatant; and desalinating said first solids fraction and said second supernatant, either before or after said combining. 17/map

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