C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 5/08 (2006.01)
Patent
CA 2648327
Provided are improved methods for tissue engineering. More particularly, methods for inducing differentiation of human embryonic stem cells into chondrocytes for use in tissue engineering applications are provided. One example of a method is a method for inducing differentiation of human embryonic stem cells into chondrocytes comprising aggregating undifferentiated human embryonic stem cells to form embryoid bodies; and culturing the embryoid bodies in culture medium in the presence of growth factors that induce chondrogenic differentiation of the embryoid bodies.
L'invention concerne des procédés d'ingéniérie tissulaire, en particulier des procédés destinés à induire une différenciation de cellules souches embryonnaires humaines en chondrocytes pour l'utilisation en ingéniérie tissulaire. Dans un mode de réalisation, le procédé visant à induire une différenciation de cellules souches embryonnaires humaines en chondrocytes consiste à agréger des cellules souches embryonnaires humaines non différenciées pour former des corps embryoïdes, et à cultiver ces corps embryoïdes dans un milieu de culture en présence de facteurs de croissance qui induisent une différenciation chondrogénique des corps embryoïdes.
Athanasiou Kyriacos A.
Hoben Gwendolyn
Hu Jerry
Koay Eugene J.
Gowling Lafleur Henderson Llp
William Marsh Rice University
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