Tissue sample preprocessing methods and devices

Details Tissue sample preprocessing methods and devices Tissue sample preprocessing methods and devices

G - Physics

01

N

G01N 1/28 (2006.01) C12M 3/00 (2006.01) C12M 3/04 (2006.01) G01N 33/48 (2006.01)

Patent

CA 2716251

To preprocess a tissue sample, a first permeable mesh membrane is tautly stretched out over the tissue sample and overlaid on top of a flat surface. This covering sandwiches and secures the tissue sample in between the first permeable mesh membrane and the flat surface. If it is desired to reinforce the mechanical strength of the first permeable mesh membrane, a second permeable mesh membrane can be tautly stretched out and overlaid on top of the sandwich. Alternatively, the second permeable mesh membrane can be attached onto a third component, such as a disposable solvent resistant frame, to create a framed mesh. Thereafter, the first permeable mesh membrane may be attached to the framed mesh to create a compound framed mesh. The tissue sample may then be sand-wiched and secured in between the compound framed mesh and the flat surface. To retain and flatten the tissue sample, gentle pressure may be uniformly applied.

Selon l'invention, pour pré-traiter un échantillon de tissu, une première membrane en treillis perméable est tendue au-dessus de l'échantillon de tissu et placée sur une surface plane. Ce recouvrement prend en sandwich et fixe l'échantillon de tissu entre la première membrane en treillis perméable et la surface plane. Si l'on souhaite renforcer la résistance mécanique de la première membrane en treillis perméable, on peut tendre une deuxième membrane en treillis perméable au-dessus du sandwich. En variante, la deuxième membrane en treillis perméable peut être fixée sur un troisième composant, par exemple un bâti à usage unique résistant aux solvants, pour créer un treillis encadré. Ensuite, la première membrane en treillis perméable peut être fixée à la membrane encadrée pour former un treillis encadré composite. L'échantillon de tissu peut alors être pris en sandwich et fixé entre le treillis encadré composite et la surface plane. Pour retenir et aplatir l'échantillon de tissu, on peut appliquer uniformément une pression modérée.

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