Tissue system with undifferentiated stem cells derived from...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 5/071 (2010.01) C12N 5/074 (2010.01) A01N 1/02 (2006.01) A61K 35/12 (2006.01) A61K 35/44 (2006.01) A61P 27/02 (2006.01)

Patent

CA 2554691

The present disclosure describes a tissue system with self-regenerating limbal stem cells, wherein the limbal stem cells are primarily undifferentiated stem cells (USCs). The tissue system is derived from isolated corneal limbal tissue, and is suitable for restoring ocular surface impairments, particularly those that result from limbal stem cell deficiencies. The tissue system is generated by selectively augmenting the tissue system for USCs, for example by selecting and sorting cells that express stem cell-specific surface markers, such as stage specific embryonic antigen marker 4 (SSEA-4). After isolation, the USCs are cultured on a tissue base in the presence of enriched medium to generate the tissue system, which is suitable for transplantation, implantation, or grafting.

L'invention concerne un système tissulaire comprenant des cellules souches limbiques auto-régénérantes, ces cellules souches limbiques étant principalement des cellules souches non différenciées. Ce système tissulaire est dérivé d'un tissu isolé du limbe cornéen, et convient pour la restauration d'une surface oculaire présentant des dégradations, en particulier des dégradations causées par une insuffisance des cellules souches limbiques. On produit ce système tissulaire par une augmentation sélective du système tissulaire en cellules souches non différenciées, par exemple en sélectionnant et en triant les cellules qui expriment des marqueurs de surface spécifiques des cellules souches, tels que le marqueur SSEA-4 (stage specific embryonic antigen marker 4 : marqueur antigénique embryonnaire 4 spécifique du stade). Une fois isolées, les cellules souches non différenciées sont mises en cultures sur une base tissulaire en présence d'un milieu enrichi de manière à produire le système tissulaire, lequel se prête à une transplantions, à une implantation ou à une greffe.

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