Titration method for a complex viral composition

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/70 (2006.01) C12Q 1/06 (2006.01) G01N 33/569 (2006.01) G01N 33/577 (2006.01)

Patent

CA 2285647

The invention concerns a method for determining, in a composition containing different species of types of live viruses, the amount of viruses of each type or species, consisting in the following steps: propagating viruses of each type or species on cells permissive to the viruses but not inducing viral interference; assaying each type or species of virus using a specific monoclonal antibody.

L'invention a pour objet une méthode pour déterminer, dans une composition contenant différentes espèces ou types de virus vivants, la quantité de virus de chacun des types ou espèces de virus, comprenant les étapes suivantes: propagation des virus de chaque type ou espèce sur des cellules permissives aux virus mais n'induisant pas d'interférence virale, dosage de chaque type ou espèce de virus au moyen d'un anticorps monoclonal spécifique.

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