Tmb formulation for soluble and precipitable hrp-elisa

Details Tmb formulation for soluble and precipitable hrp-elisa Tmb formulation for soluble and precipitable hrp-elisa

G - Physics

01

N

G01N 33/531 (2006.01) C12Q 1/28 (2006.01) G01N 33/543 (2006.01)

Patent

CA 2045522

ABSTRACT Solvent systems for use in increasing the solubility of 3,3',5',5'-tetramethylbenzidine (TMB) and for making a permanent color record of the results of ELISAs using HRP have been developed. In the improved solvent system containing povidone, 1-ethenyl-2-pyrrolidinone polymers, TMB can be dissolved in a very small quantity of solvent, up to a concentration of 100 to 200 mM, which can then be mixed directly into an aqueous buffer without precipating the TMB. In the solvent system for making a permanent record, alginic acid (AA), methyl vinyl ether/maleic anhydride copolymer (MVE/MAC), dextran sulfate (DS) and/or carrageenan are added to the aqueous buffer solution, with or without povidone, to form a colored precipate with the reaction product.

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