Tolerance-induced targeted antibody production

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 5/18 (2006.01) A61K 39/00 (2006.01) C07K 14/705 (2006.01) C07K 16/30 (2006.01) C12N 5/02 (2006.01) C12P 21/08 (2006.01)

Patent

CA 2514177

The present invention provides methods for directing the immune response of an animal towards immunologically weak or rare antigens such as tumor antigens. The methods combine subtractive immunization with hyperimmunization and result in the controlled or directed production of target-specific antibodies, helper T cells (CD4+-T lymphocytes) and cytotoxic T cells (CD8+-T lymphocytes). Also provided by the present invention are untransformed and transformed cell lines, and growth media necessary to grow the untransformed cell line in a differentiated state. Monoclonal antibodies which react with different neoplastic cell lines and hybridomas producing such antibodies are also provided.

La présente invention a trait à des procédés pour la délivrance d'une réponse immunitaire d'un animal dans des antigènes immunologiquement faibles ou rares tels que des antigènes tumoraux. Les procédés réalisent la combinaison de l'immunisation soustractive avec l'hyperimmunisation et entraînent la production contrôlée ou orientée d'anticorps ciblés, de lymphocytes T auxiliaires (lymphocytes T CD4?+¿) et de cellules T cytotoxiques (lymphocytes T CD8?+¿). L'invention a également trait à des lignées cellulaires non transformées et transformées, des milieux de croissance nécessaires à la croissance de la lignée cellulaire non transformée dans un état différencié. L'invention a trait en outre à des anticorps monoclonaux qui réagissent avec différentes cellules néoplasiques et des hybridomes produisant de tels anticorps.

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