Tomato plants having higher levels of resistance to botrytis

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/82 (2006.01) A01H 5/08 (2006.01)

Patent

CA 2650465

The present invention relates to a method for detecting a quantitative trait locus (QTL) associated with resistance to Botrytis cinerea in tomato, comprising the steps of crossing a Botrytis-resistant donor tomato plant with a non-resistant, or Botrytis-susceptible, recipient tomato plant, contacting one or more offspring plants with an infective amount of Botrytis, quantitatively determining the disease incidence and/or the rate of lesion growth in said one or more offspring plants, establishing a genetic linkage map that links the observed disease incidence and/or rate of lesion growth to the presence of chromosomal markers of said donor tomato plant in said one or more offspring plants, and assigning to a QTL the contiguous markers on said map that are linked to a reduced disease incidence and/or a reduced lesion growth rate.

La présente invention concerne un procédé permettant de détecter un locus quantitatif (QTL), associé à la résistance à la Botrytis cinerea chez la tomate, qui comprend les étapes consistant à : croiser un plant de tomates donneur résistant à la Botrytis avec un plant de tomates récepteur ne résistant pas ou sensible à la Botrytis ; mettre en contact un ou plusieurs plants qui sont issus de ce croisement avec une quantité infectieuse de Botrytis ; déterminer de manière quantitative l'incidence de la maladie et/ou le taux de croissance de la lésion dans ledit ou lesdits plants issus du croisement ; établir une carte de liaison génétique qui lie l'incidence de la maladie et/ou le taux de croissance de la lésion observés en présence de marqueurs chromosomiques dudit plant de tomates donneur dans ledit ou lesdits plants de tomates issus du croisement ; et attribuer à un locus quantitatif les marqueurs contigus sur ladite carte qui sont liés à une incidence réduite de la maladie et/ou un taux de croissance réduit de la lésion.

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