Tool for quantitative real-time analysis of viral gene...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) C12Q 1/70 (2006.01)

Patent

CA 2630353

Tool for quantitative real-time analysis of viral gene expression dynamics in single living cells The invention provides a method allowing the detection and the quantitative real-time measurement, at the single living cell level, of viral replication using a bioluminescent reporter gene and a digital light detection device sensitive to detect single photons with high efficiency and assign them a lateral x,y coordinate and precise temporal incidence (i.e. a "time point"), wherein the spatial and temporal characteristics of bioluminescence is indicative at the single living cell level of viral replication.

L'invention concerne un outil d'analyse quantitative en temps réel de la dynamique d'expression de gènes viraux dans des cellules vivantes isolées. L'invention concerne un procédé qui permet la détection et la mesure quantitative en temps réel de la réplication virale au niveau de la cellule vivante isolée en utilisant un gène reporteur bioluminescent et un dispositif numérique de détection de lumière suffisamment sensible pour détecter à haut rendement des photons individuels et leur attribuer des coordonnées x, y latérales et une incidence temporelle précise (c'est-à-dire un "instant"), les caractéristiques spatiales et temporelles de la bioluminescence étant une indication de la réplication virale au niveau de la cellule vivante isolée.

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