C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01)
Patent
CA 2697640
The present invention provides tools and methods for use in genetic tests involving high performant sequencing techniques. More particularly, the invention provides a robust multiplex PCR method wherein the respective primers for amplifying the different amplicons are physically isolated from one another. The invention further provides quality control methods allowing a stringent monitoring of genetic tests carried out according to the present invention.
La présente invention concerne des outils et des procédés utilisables dans des tests génétiques impliquant des techniques de séquençage hautement performantes. Plus précisément, l'invention concerne un procédé efficace de PCR multiplexe dans lequel les amorces respectives d'amplification des différents amplicons sont physiquement isolées les unes des autres. L'invention concerne, en outre, des procédés de contrôle qualité permettant un suivi rigoureux des tests génétiques mis en uvre conformément à la présente invention.
Katholieke Universiteit Leuven
Smart & Biggar
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