Total amino acid stabilization during cell-free protein...

Details Total amino acid stabilization during cell-free protein... Total amino acid stabilization during cell-free protein...

C - Chemistry, Metallurgy

12

P

C12P 21/06 (2006.01)

Patent

CA 2611908

Compositions and methods are provided for the enhanced in vitro synthesis of protein molecules, by optimizing the metabolism of amino acids present in the reaction mix, preferably all amino acids in the reaction mixture. By performing synthesis with extracts from genetically modified microbial strains that are deficient in multiple amino acid metabolizing enzymes reduces the enzymatic activities responsible for catalyzing these reactions and improves the overall yield of synthesis.

L'invention concerne des compositions et des méthodes pour la synthèse in vitro améliorée de molécules protéiques par optimisation du métabolisme d'acides aminés présents dans le mélange de réaction, et de préférence de tous les acides aminés présents dans le mélange de réaction. La réalisation d'une synthèse avec des extraits issus de souches microbiennes génétiquement modifiées présentant une carence en diverses enzymes de métabolisation des acides aminés permet de réduire les activités enzymatiques responsables de la catalyse de ces réactions et d'améliorer le rendement global de la synthèse.

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