Totally synthetic affinity reagents

Details Totally synthetic affinity reagents Totally synthetic affinity reagents

C - Chemistry, Metallurgy

12

N

C12N 15/62 (2006.01) C07K 16/00 (2006.01) C07K 16/18 (2006.01) C07K 16/30 (2006.01) C07K 16/40 (2006.01) C07K 16/44 (2006.01) C07K 19/00 (2006.01) C12N 15/10 (2006.01) C12N 15/70 (2006.01)

Patent

CA 2075974

A novel process for producing novel and/or improved heterofunctional binding fusion proteins termed Totally Synthetic Affinity Reagents (TSARs) is disclosed. TSARs are concatenated heterofunctional polypeptides or proteins comprising at least two functional regions: a binding domain with affinity for a ligand and a second effector peptide portion that is chemically or biologically active. In one embodiment, the heterofunctional polypeptides or proteins further comprise a linker peptide portion between the binding domain and the second active peptide portion. The linker peptide can be either susceptible or not susceptible to cleavage by enzymatic or chemical means. Novel and/or improved heterofunctional binding reagents as well as methods for using the reagents for a variety of in vitro and in vivo applications are also disclosed.

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