Toxicity testing assay

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/18 (2006.01) C12M 1/34 (2006.01) G01N 33/18 (2006.01)

Patent

CA 2134636

A toxicity testing assay wherein a test sample is prepared with electroceramic dye and a living organism of the cladoceran order or other test organisms, and the sample is irradiated with alternately blue and yellow light to excite fluorescence. The successive groups of fluorescent emissions are then viewed at 90° by a electroceramic which develops equivalent alternate count outputs, and the count output is amplified and processed to develop data indicating (1) membrane potential of cells of the living organisms and (2) the total dye fluorescence which provides indication of deleterious effects to the organisms.

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