Trait-associated gene identification method

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/10 (2006.01) C12N 5/04 (2006.01) C12N 5/10 (2006.01) C12N 15/82 (2006.01) C12Q 1/02 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2364275

The present invention provides a method for identifying and characterizing genes, the modified expression of which is associated with a desired trait in a fruit-bearing plant. The method includes the steps of: transforming plant cells with a plant cell expression vector comprising an element which functions to enhance gene expression and has the ability to integrate into the plant genome in a manner effective to enhance expression of native plant genes, selecting transformed plant cells, regenerating transformed plant cells to yield mature plants, selecting plants having a desired trait, identifying, isolating and characterizing genes the transcription of which has been enhanced and confirming the contribution of the modified expression of each identified gene to the desired trait.

La présente invention concerne une méthode d'identification et de caractérisation de gènes, dont l'expression modifiée est associée à un caractère voulu dans un plant fruitier. Cette méthode consiste à: transformer des cellules végétales avec un vecteur d'expression de cellule végétale comportant un élément qui fonctionne de façon à améliorer l'expression génique, et capable de s'introduire dans le génome de la plante de manière efficace permettant d'améliorer l'expression de gènes de plantes natifs, sélectionner ensuite les cellules de plantes végétales transformées, régénérer les cellules de plantes transformées pour produire des plantes matures, puis sélectionner des plantes présentant un caractère choisi, identifier, isoler, et caractériser des gènes dont la transcription a été améliorée, et enfin, confirmer la contribution de l'expression de chaque gène identifié au caractère voulu.

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