Transcription factor modulating compounds and methods of use...

A - Human Necessities – 61 – K

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A61K 31/553 (2006.01) A61K 31/00 (2006.01) A61K 31/519 (2006.01) A61L 2/16 (2006.01) A61L 12/08 (2006.01) A61P 31/04 (2006.01) C12Q 1/02 (2006.01) C12Q 1/18 (2006.01) C12Q 1/68 (2006.01) G06F 17/00 (2006.01) G06F 19/00 (2006.01)

Patent

CA 2445515

Methods for identifying compound useful as anti-infectives that decrease resistance, virulence, or growth of microbes are provided. In one embodiment, the method comprises contacting a microbial cell comprising: 1) a selectable marker under the control of a transcription factor responsive element and 2) a transcription factor, with a compound under conditions which allow interaction of the compound with the microbial cell; and measuring the ability of the compound to affect the growth or survival of the microbial cell as an indication of whether the test compound modulates the activity of a transcription factor.

La présente invention concerne des procédés qui permettent d'identifier un composé destiné à être utilisé comme anti-infectieux qui réduit la résistance, la virulence ou la croissance des microbes. Dans un mode de réalisation, le procédé consiste à mettre en contact une cellule microbienne comprenant : 1) un marqueur sélectionnable sous le contrôle d'un élément sensible à un facteur de transcription, et 2) un facteur de transcription, avec un composé dans des conditions qui permettent une interaction du composé avec la cellule microbienne ; et à mesurer la capacité du composé à influencer la croissance ou la survie de la cellule microbienne, ladite capacité indiquant si le composé à l'essai module l'activité d'un facteur de transcription.

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