Transcription-free selex

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) C07H 21/04 (2006.01) C12P 19/34 (2006.01)

Patent

CA 2391351

Methods are provided for the production of nucleic acid ligands against target molecules using a procedure known as Transcription-free Systematic Evolution of Ligands by EXponential enrichment (Transcription-free SELEX). The transcription-free SELEX method assembles nucleic acid ligands from fragments of synthetic nucleic acids by annealing those fragments to a complementary template, and then ligating the fragments together.

L'invention concerne des procédés de production de ligands d'acides nucléiques contre des molécules cibles par l'évolution systématique de ligands par enrichissement exponentiel sans transcription (procédé SELEX sans transcription). Ledit procédé permet d'assembler des ligands d'acides nucléiques à partir de fragments d'acides nucléiques synthétiques par annelage desdits fragments à un modèle complémentaire et de les ligaturer.

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