Transcriptional regulatory sequence of carcinoembryonic...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/86 (2006.01) A61K 31/70 (2006.01) A61K 35/76 (2006.01) A61K 38/50 (2006.01) A61K 48/00 (2006.01) C12N 15/55 (2006.01) C12N 15/85 (2006.01) C12N 15/867 (2006.01)

Patent

CA 2176014

The invention relates to the transcriptional regulatory sequence (TRS) of carcinoembryonic antigen (CEA) and molecular chimaera comprising the CEA TRS and DNA encoding a heterologous enzyme. CEA TRS is capable of targeting expression of the heterologous enzyme to CEA+ cells and the heterologous enzyme is preferably an enzyme capable of catalysing the production of an agent cytotoxic or cytostatic to CEA+ cells. For example the enzyme may be cytosine deaminase which is capable of catalysing formation of the cytotoxic compound 5-fluorouracil from the non toxic compound 5-fluorocytosine.

L'invention concerne une séquence régulatrice de transcription propre à un antigène carcinoembryonnaire ainsi qu'une chimère moléculaire comprenant cette séquence d'antigène et l'ADN codant une enzyme hétérologue. Cette séquence d'antigène peut cibler l'expression de l'enzyme hétérologue sur des cellules?+¿ d'antigène carcinoembryonnaire et l'enzyme hétérologue peut de préférence catalyser la production d'un agent cytotoxique ou cytostatique de ces cellules?+¿ d'antigène carcinoembryonnaire. Cette enzyme peut être par exemple une cytosine désaminase pouvant catalyser la formation d'un composé toxique, le 5-fluorouracile, à partir d'un composé non toxique, la 5-fluorocytosine.

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