Transduction of primary cells

Details Transduction of primary cells Transduction of primary cells

C - Chemistry, Metallurgy

12

N

C12N 15/867 (2006.01) A61K 48/00 (2006.01)

Patent

CA 2606928

Methods, compositions, and systems relating to the stable transduction of primary cells of the hematopoietic system and/or hematopoietic stem cells. A method for stable transduction of primary cells of the hematopoietic system and/or hematopoietic stem cells, comprising contacting, in vitro or ex vivo, the surface of the cells with both a lentiviral vector and at least one molecule which binds the cell surface, and culturing the cells in a ventilated vessel comprising two or more layers under conditions conducive to growth and/or proliferation, wherein the vessel is suitable for culturing at least about 100 million cells. Use of the transduced primary cells to treat, diagnose, alleviate or prevent a tumor or infection in a subject is also disclosed. A system comprising a vessel or flask to grow the primary cells in is also described.

L'invention concerne des procédés, des compositions, et des systèmes associés à la transduction stable de cellules primaires du système hématopoïétique et/ou de cellules souches hématopoïétiques. L'invention concerne un procédé de transduction stable de cellules primaires du système hématopoïétique et/ou de cellules souches hématopoïétiques qui consiste : à mettre en contact, in vitro ou ex vivo, la surface des cellules avec un vecteur lentiviral et au moins une molécule qui se lie à la surface de la cellule ; et à cultiver les cellules dans une cuve ventilée comprenant au moins deux couches dans des conditions induisant une croissance et/ou une prolifération, la cuve étant apte à être utilisée pour cultiver au moins environ 100 millions de cellules. L'invention concerne également l'utilisation de cellules primaires transduites pour traiter, diagnostiquer, soulager ou prévenir une tumeur ou une infection chez un sujet. L'invention concerne en outre un système comprenant une cuve ou un flacon pour la culture de cellules primaires.

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