Transfected cell lines expressing autoantigens and their use...

Details Transfected cell lines expressing autoantigens and their use... Transfected cell lines expressing autoantigens and their use...

C - Chemistry, Metallurgy

12

N

C12N 5/10 (2006.01) C07K 14/47 (2006.01) C12Q 1/02 (2006.01) G01N 33/564 (2006.01)

Patent

CA 2175478

This invention relates to improved in vitro immunoassay methods for detection of autoantibodies associated with autoimmune disease. More specifically the invention relates to immunoassay methods which utilize a human cell line stably transformed with a nucleic acid expressing the autoimmune antigens 52 or 60kd Ro/SS-A or La/SS-B. This invention also relates to compositions comprising these cell lines and to kits containing such cell lines.

L'invention se rapporte à des méthodes de dosage immunologique in vitro améliorées, destinées à la détection d'autoanticorps associées aux maladies autoimmunes. L'invention se rapporte plus particulièrement à des méthodes de dosage immunologique consistant à utiliser une lignée cellulaire humaine transformée de façon stable au moyen d'un acide nucléique exprimant les antigènes autoimmuns 52 ou 60 kd Ro/SS-A ou La/SS-B. Cette ivention se rapporte également à des compositions comprenant ces lignées cellulaires, ainsi qu'à des matériels contenant ces dernières.

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