Transfer of macromolecules from a chromatographic substrate...

Details Transfer of macromolecules from a chromatographic substrate... Transfer of macromolecules from a chromatographic substrate...

B - Operations, Transporting

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252/31, 167/44,

B01D 15/08 (2006.01) B01D 57/02 (2006.01) B01D 67/00 (2006.01) B01D 71/56 (2006.01) B01J 20/26 (2006.01) B01J 20/32 (2006.01) G01N 33/545 (2006.01)

Patent

CA 1207737

TRANSFER OF MACROMOLECULES FROM A CHROMATOGRAPHIC SUBSTRATE TO AN IMMOBILIZING MATRIX ABSTRACT OF THE DISCLOSURE The method of transfer of macromolecules such as nucleic acid and proteins from a chromatographic substrate to an immobilizing matrix uses as the immobilizing matrix, a charge modified mioroporous membrane comprising an or- ganic microporous membrane having a charge modifying amount of a cationic charge modifying agent bonded to substantially all of the wetted surfaces of said membrane. The charge modified microporous membrane can also be a reinforced microporous membrane, preferably a porous reinforcing web impregnated with a polymeric microporous membrane. A nucleic acid or protein blotting product comprising a chromatographic matrix having the charge modified micro- porous membrane on a surface thereof is also provided.

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