Transferases, epimerases, polynucleotides encoding these and...

C - Chemistry – Metallurgy – 07 – K

Patent

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C07K 4/10 (2006.01) A01H 3/00 (2006.01) A01N 61/00 (2006.01) C07K 7/08 (2006.01) C07K 14/415 (2006.01) C12N 5/14 (2006.01) C12N 15/29 (2006.01)

Patent

CA 2680196

The invention provides compositions and methods for modulating GDP-L-Galactose Guanyltransferase (also known as GDP-L-Galactose phosphorylase) activity; and/or GDP-D-Mannose epimerase activity; and/or ascorbate content in plants. The invention provides plants and plant cells with increased GDP-L-Galactose Guanyltransferase activity; and/or GDP-D-Mannose epimerase activity. The invention provides plants and plant cells with increased ascorbate content as a result of: over-expression of GDP-L-Galactose Guanyltransferase; over-expression of GDP-D-Mannose epimerase; or in particular over-expression of a combination of GDP-L-Galactose Guanyltransferase and GDP-D-Mannose epimerase.

L'invention concerne des compositions et des méthodes pour moduler l'activité GDP-L-galactose guanyltransférase (également connue sous le nom de GDP-L-galactose phosphorylase) et/ou l'activité GDP-D-mannose épimerase et/ou la teneur en ascorbate dans les plantes. L'invention concerne des plantes et des cellules végétales présentant une activité GDP-L-galactose guanyltransférase et/ou une activité GDP-D-mannose épimerase accrue. L'invention a pour objet des plantes et des cellules végétales ayant une teneur accrue en ascorbate suite à une surexpression de la GDP-L-galactose guanyltransférase, à une surexpression de la GDP-D-mannose épimérase ou notamment suite à la surexpression d'une combinaison de la GDP-L-galactose guanyltransférase et de la GDP-D-mannose épimerase.

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