Transformation method and transgenic plants produced thereby

Details Transformation method and transgenic plants produced thereby Transformation method and transgenic plants produced thereby

C - Chemistry, Metallurgy

12

N

C12N 15/90 (2006.01) A01H 5/00 (2006.01) C12N 5/04 (2006.01) C12N 15/29 (2006.01) C12N 15/63 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2379076

This invention relates to methods for producing, at a high frequency, transgenic plants that contain little if any vector sequences, have simple integration patterns, contain few copies of the transgene at each locus, express the transgene at all stages of development and do not exhibit transgene silencing. The method comprises introducing minimal transgene expression cassettes, which are substantially or totally devoid of vector sequences, by direct DNA transfer, preferably by particle or microprojectile bombardment. This invention also relates to transformed plant cells, the transgenic plants regenerated therefrom, and subparts of the transgenic plants produced by the methods of this invention. The invention also includes all progeny and subsequent progeny (i.e., all subsequent generations) derived from primary transformants through selfing or crossing.

Cette invention a trait à des méthodes permettant de produire, à une cadence élevée, des plantes transgéniques contenant, éventuellement, de petites séquences de vecteurs, ayant des configurations d'intégration simples, contenant quelques copies du transgène à chaque locus, exprimant le transgène à tous les stades du développement et ne faisant pas montre de suppression du transgène. La méthode consiste à introduire des cassettes d'expression transgénique minimale, qui sont quasiment ou totalement exemptes de séquences de vecteurs, par transfert direct d'ADN, de préférence par bombardement de particules ou de microprojectiles. Cette invention a également trait à des cellules végétales transformées, à des plantes transgéniques régénérées à partir de celles-ci, et à des sous-parties des plantes transgéniques produites grâce aux méthodes selon l'invention. Elle porte, en outre, sur toute la descendance et la descendance ultérieure (ces à dire toutes les générations à venir) dérivée des premiers transformants par conjugaison intractonale ou croisement.

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